Wednesday, June 30, 2021

AM Normal Cell Free gDNA

 

SARS-CoV-2 reference Standard and Quality Control

AM Normal cfgDNA-1
Description: DNA is extracted and fragmented from a popular normal male cell line

Dissolved in TE: Part Number: 60133001
10ug per vial
Spiked in Plasma: Part Number: 60133501
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDNA-2
Description: DNA is extracted and fragmented from normal female cell line, PAM3005
Dissolved in TE: Part Number: 60133002
10ug per vial
Spiked in Plasma: Part Number: 60133502
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDNA-3
Description: DNA is extracted and fragmented from pooled normal peripheral blood mononuclear cells (PBMC) from fresh blood
Dissolved in TE: Part Number: 60133003
10ug per vial
Spiked in Plasma: Part Number: 60133503
Pre-spiked with 250ng gDNA per 5ml

Order HereSARS-CoV-2 reference Standard and Quality Control

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

AM Variant Cell Free gDNA

 

SARS-CoV-2 reference Standard and Quality Control

AM Multiplexed ctDNA
Description: DNA contains multiplexed 20+ cell-derived variants each at 5% Allele Frequency. DNA is extracted and fragmented from mutated PAM3005 cells

     Dissolved in TE: Part Number: 60135001
     10ug per vial
     Spiked in Plasma: Part Number: 60135501
     Pre-spiked with 250ng gDNA per 5ml

Variants included

APC Q1429*KIT D816V
APC R1450*KRAS G12C
BRAF V600EKRAS G12D
CTNNB1 T41AKRAS G12V
EGFR C797SNRAS Q61R
EGFR E746-A750delPIK3CA E545K
EGFR L858RPIK3CA H1047R
EGFR D770_N771insGPTEN R130G
EGFR T790MTP53 R175H
FGFR3 S249CTP53 R248Q
IDH1 R132H

AM Trisomy cfgDNA
Description: DNA is extracted and fragmented from cells harboring trisomy 13, 18 or 21. It is used for PCR- or counting-based NIPT assay
      Dissolved in TE: Part Numbers: 60134001 (Trisomy13), 60134002 (Trisomy18), 60134003 (Trisomy21)
     10ug per vial
     Spiked in Plasma: Part Numbers: 60134501 (Trisomy13), 60134502 (Trisomy18), 60134503 (Trisomy21)
     Pre-spiked with 250ng gDNA per 5ml

Order HereSARS-CoV-2 reference Standard and Quality Control

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

Thursday, June 24, 2021

DNA-Depleted Plasma

 

DNA-Depleted Plasma

Build your own controls using your high-positive samples to save time. With our DNA-depleted human plasma (DNA-free plasma) as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample.

DNA-Depleted Plasma

Prefer to have a knowledgeable lab do the work? Send your plasma to Anchor Molecular and we’ll remove the DNA background for you.

Product NameCat. No.Size
DD Plasma (DNA-Depleted Plasma)6060100150, 200, 500, 1000 ml
DD Serum (DNA-Depleted Serum)6060100250, 200, 500, 1000 ml

Why Is Plasma Better Than Buffer As Reference Material Matrix?

A plasma matrix is a better representation of a real patient sample because the natural, interfering proteins are retained. This allows for a more realistic process in your quality system.

DNA- free depleted plasma



Equal amounts of DNA fragments were spiked into TE Buffer (blue) and human plasma (red).

While the 170 bp peak was recovered similarly in each matrix, much more genomic DNA was extracted from the plasma than from the TE buffer resulting in a more patient-like matrix.
See Figure A.

 

Exosome counts in DNA-depleted plasma are normal compared to regular plasma.

Exosome
DNA- free depleted plasma

 

AM DNA-Depleted Plasma

  • Highest quality DNA-depleted human plasma
  • For use in research, assay development, & daily assays
  • Retains naturally-occurring, interfering proteins
  • Allows for more realistic process in quality systems
  • Customized plasma available upon request
  • Price upon request

Order HereDNA- free depleted plasma

CHEMICAL PRODUCT CHARACTERISTICS
Concentration of DNA:  < 10 pg/mL
Total protein: 5-7 g/dL
Immunoglobulin G: 500-1700 mg/dL
Immunoglobulin A: 90-400 mg/dL
Immunoglobulin M: 20-172 mg/dL
Cholesterol, Total:  50-199 mg/dL
Triglycerides: 50-149 mg/dL
HDL Cholesterol:  > 20 mg/dL
VLDL Cholesterol: 10-40 mg/dL
LDL Cholesterol:  20-99 mg/dL
pH: 7.2-7.6
Tested Negative for HIV, HBV, HCV,
 HTLV and Syphilis by FDA approved methods

ctDNA Controls & Reference Standards

 

ctDNA Controls & Reference Standards

cfDNA reference Standard and Quality Control


Buffer-based or plasma-based controls are produced by mixing single or multiplexed mutant gene fragments with a native, whole-genome cfDNA background (Figure B).

Anchor Molecular manufactures two types of products: Mutation Quantification Control and Mutation Reference Standard. The user can quantify either the absolute copy numbers or the relative number of the mutant gene fragments.

AM Multiplexed Mutation Quantification Control

  • Function as both positive and negative controls
  • For use in daily assays
  • AF% determined based on average wild-type copy numbers
  • Customized controls available upon request
  • Price upon request

This product is used as Positive and Negative Controls in daily assays. The AF% is determined based on average wildtype copy numbers. It includes all mutant genes listed in the table below. Custom products with different parameters are available upon request.

cfDNA reference Standard and Quality Control

Cat. No.Base Matrix (vol)Allele Frequency (%)Wildtype gDNA (ng)
60100101Buffer (25 ul)0 or 2250
60100102EDTA Plasma (5 ml)0 or 250
60100103EDTA Plasma (5 ml)0 or 225

AM Multiplexed Mutation Reference Standard

  • Highest quality reference standards
  • For use in research and assay development
  • Mutant genes at fixed allele frequency (AF%) determined by ddPCR
  • Customized reference standards available upon request
  • Price upon request

Mutant genes are made at fixed allele frequency (AF%) determined by ddPCR. The product includes all mutant 37 mutant genes listed in red in the table below. Custom products with different parameters are available upon request.

Cat. No.Base Matrix (vol)Allele Frequency (%)Wildtype gDNA (ng)
60100201Buffer (25 ul)0, 0.1, 0.3, 1, or 2250
60100202EDTA Plasma (5 ml)0, 0.1, 0.3, 1, or 2125

Order Here

Mutation List  

EGFR (T790M)KRAS (G12S)TP53 (R175H)EGFR (D770-N771insG)
EGFR (C797S)KRAS (G12A)APC (R1450X)EGFR (delE746-A750)
EGFR (T790M & C797S cis)KRAS  (G12R)Beta2-MEGFR (delL747-T751insS)
EGFR (G719S)KRAS (G13D)DDR2EGFR (delL747-P753insS)
EGFR (G719C)BRAF (V600E)LKB1
EGFR (G719A)BRCA1 (P871L)MDM2
EGFR (S720F)BRCA2 (N372H)MDM4EML4-ALK v1
EGFR (L858R)ERBB2 (S310F)POLEEML4-ALK v2
EGFR (L861Q)JAK2 (V617F)PTENEML4-ALK v3
EGFR (L861R)MAP2K1 (P124S)SKT11CCDC6-RET
KRAS (G12D)MET (Y1253D)NCOA4-RET
KRAS (G12V)PIK3CA (H1047R)EGFR (D770-N771insNPG)CD74-ROS1
KRAS (G12C)TERT (A305A)EGFR (D770-N771insSVQ)SLC34A2-ROS1

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